Western blot was performed using Anti-FXN Monoclonal Antibody (18A5DB1) (Product # 45-6300) and a 14 kDa band corresponding to Frataxin, mitochondrial was observed across cell lines tested. Membrane enriched extracts (30 µg lysate) of Jurkat (Lane 1), A-431 (Lane 2) and HeLa (Lane 3) were electrophoresed using Novex™ 16% Tricine Protein Gel (Product # EC6695BOX). Resolved proteins were then transferred onto a Nitrocellulose membrane (Product # IB23001) by iBlot® 2 Dry Blotting System (Product # IB21001). The blot was probed with the primary antibody (3 µg/mL) and detected by chemiluminescence with Goat anti-Mouse IgG (H+L) Superclonal™ Recombinant Secondary Antibody, HRP (Product # A28177, 1:4000 dilution) using the iBright FL 1000 (Product # A32752). Chemiluminescent detection was performed using Novex® ECL Chemiluminescent Substrate Reagent Kit (Product # WP20005).